J Biol Chem. 2001 Apr 13;276(15):11877-82. Epub 2001 Jan 12.

CARD11 and CARD14 are novel caspase recruitment domain (CARD)/membrane-associated guanylate kinase (MAGUK) family members that interact with BCL10 and activate NF-kappa B.External

Bertin, J., Wang, L., Guo, Y., Jacobson, M. D., Poyet, J. L., Srinivasula, S. M., Merriam, S., DiStefano, P. S., Alnemri, E. S.,
--- - Millennium Pharmaceuticals, Inc., Cambridge, Massachusetts 02139, USA.
The caspase recruitment domain (CARD) is a protein-binding module that mediates the assembly of CARD-containing proteins into apoptosis and NF-kappaB signaling complexes. We report here that CARD protein 11 (CARD11) and CARD protein 14 (CARD14) are novel CARD-containing proteins that belong to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. CARD11 and CARD14 have homologous structures consisting of an N-terminal CARD domain, a central coiled-coil domain, and a C-terminal tripartite domain comprised of a PDZ domain, an Src homology 3 domain, and a GUK domain with homology to guanylate kinase. The CARD domains of both CARD11 and CARD14 associate specifically with the CARD domain of BCL10, a signaling protein that activates NF-kappaB through the IkappaB kinase complex in response to upstream stimuli. When expressed in cells, CARD11 and CARD14 activate NF-kappaB and induce the phosphorylation of BCL10. These findings suggest that CARD11 and CARD14 are novel MAGUK family members that function as upstream activators of BCL10 and NF-kappaB signaling.
PMID: 11278692External
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
CARD Bcl-10 Link CARD11 GST fusion protein pull-down Not specified Not specified E.coli Not specified 1-126 In vitro translation 11278692
CARD Bcl-10 Link CARD11 Two-hybrid Not specified Not specified HEK293 Not specified 1-126 HEK293 11278692
CARD Bcl-10 Link CARD14 GST fusion protein pull-down Not specified Not specified E.coli Not specified 1-118 In vitro translation 11278692
CARD Bcl-10 Link CARD14 Two-hybrid Not specified Not specified HEK293 Not specified 1-118 HEK293 11278692
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
CARD Bcl-10 Link CARD11 Co-immunoprecipitation HEK293 Not used Not specified 1-126 11278692
CARD Bcl-10 Link CARD14 Co-immunoprecipitation HEK293 Not used Not specified 1-118 11278692
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
CARD Bcl-10 Link CARD11 Luciferase assay NF-kB activation (signalosome) 11278692
CARD Bcl-10 Link CARD14 Luciferase assay NF-kB activation (signalosome) 11278692
(Link: click this icon to show interactions only between the two corresponding DDs)