Biochem Biophys Res Commun. 2001 Jun 1;284(1):77-82.

Human CARD12 is a novel CED4/Apaf-1 family member that induces apoptosis.External

Geddes, B. J., Wang, L., Huang, W. J., Lavellee, M., Manji, G. A., Brown, M., Jurman, M., Cao, J., Morgenstern, J., Merriam, S., Glucksmann, M. A., DiStefano, P. S., Bertin, J.,
--- - Millennium Pharmaceuticals, Inc., Cambridge, Massachusetts 02139, USA.
The CED4/Apaf-1 family of proteins functions as critical regulators of apoptosis and NF-kappaB signaling pathways. A novel human member of this family, called CARD12, was identified that induces apoptosis when expressed in cells. CARD12 is most similar in structure to the CED4/Apaf-1 family member CARD4, and is comprised of an N-terminal caspase recruitment domain (CARD), a central nucleotide-binding site (NBS), and a C-terminal domain of leucine-rich repeats (LRR). The CARD domain of CARD12 interacts selectively with the CARD domain of ASC, a recently identified proapoptotic protein. In addition, CARD12 coprecipitates caspase-1, a caspase that participates in both apoptotic signaling and cytokine processing. CARD12 may assemble with proapoptotic CARD proteins to coordinate the activation of downstream apoptotic and inflammatory signaling pathways.
PMID: 11374873External
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
CARD ASC Link CARD12 Two-hybrid Not specified Not specified HEK293 Not specified 1-83 HEK293 11374873
CARD CARD12 Link CARD12 Two-hybrid Not specified 1-83 HEK293 Not specified 1-83 HEK293 11374873
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
CARD CARD12 Link Caspase1 Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 11374873
(Link: click this icon to show interactions only between the two corresponding DDs)