J Biol Chem. 2001 Sep 14;276(37):34495-500. Epub 2001 Jun 29.

Cop, a caspase recruitment domain-containing protein and inhibitor of caspase-1 activation processing.External

Lee, S. H., Stehlik, C., Reed, J. C.,
--- - Burnham Institute, La Jolla, California 92037, USA.
The production of bio-active interleukin-1beta (IL-1beta), a pro-inflammatory cytokine, is mediated by activated caspase-1. One of the known molecular mechanisms underlying pro-caspase-1 processing and activation involves binding of the caspase-1 prodomain to a caspase recruitment domain (CARD)-containing serine/threonine kinase known as RIP2/CARDIAK/RICK. We have identified a novel protein, COP (CARD only protein), which has a high degree of sequence identity to the caspase-1 prodomain. COP binds to both RIP2 and the caspase-1 prodomain and inhibits RIP2-induced caspase-1 oligomerization. COP inhibits caspase- 1-induced IL-1beta secretion as well as lipopolysaccharide-induced IL-1beta secretion in transfected cells. Our data indicate that COP can regulate IL-1beta secretion, implying that COP may play a role in down-regulating inflammatory responses analogous to the CARD protein ICEBERG.
PMID: 11432859External
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
CARD CARD16 Link CARD16 Co-immunoprecipitation HEK293 Human Not specified Human Not specified 11432859
CARD CARD16 Link Caspase1 Co-immunoprecipitation HEK293 Human Not specified Not specified Full length 11432859
CARD CARD16 Link Caspase1 Co-immunoprecipitation HEK293 Human Not specified Not specified 1-120 11432859
CARD CARD16 Link RIPK2 Co-immunoprecipitation HEK293 Human Not specified Not specified Full length 11432859
CARD Caspase1 Link Caspase1 Co-immunoprecipitation HEK293 Not specified Full length Not specified Full length 11432859
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
CARD CARD16 Link Caspase1 Immunoprecipitation, ELISA Rip mediated procaspase1 oligomerization inhibition, IL-1b secretion decrease 11432859
(Link: click this icon to show interactions only between the two corresponding DDs)