Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):13884-8.

Caspase-10 is an initiator caspase in death receptor signaling.External

Wang, J., Chun, H. J., Wong, W., Spencer, D. M., Lenardo, M. J.,
--- - Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Building 10, Room 11N311, 10 Center Drive, MSC 1892, Bethesda, MD 20892, USA.
A role for caspase-10, previously implicated in the autoimmune lymphoproliferative syndrome, in death receptor signaling has not been directly shown. Here we show that caspase-10 can function independently of caspase-8 in initiating Fas- and tumor necrosis factor-related apoptosis-inducing ligand-receptor-mediated apoptosis. Moreover, Fas crosslinking in primary human T cells leads to the recruitment and activation of caspase-10. Fluorescent resonance energy transfer analysis indicates that the death-effector domains of caspase-8 and -10 both interact with the death-effector domain of FADD. Nonetheless, we find that caspase-8 and -10 may have different apoptosis substrates and therefore potentially distinct roles in death receptor signaling or other cellular processes.
PMID: 11717445External
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DED Caspase8 Link FADD Fluorescence resonance energy transfer ` 11717445
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DED Caspase10 Link Caspase8 Fluorescence resonance energy transfer Jurkat Not specified Full length Not specified Full length 11717445
DED Caspase10 Link FADD Fluorescence resonance energy transfer HEK293 Not specified Full length Not specified Not specified 11717445
DED Caspase8 Link Caspase8 Fluorescence resonance energy transfer Jurkat Not specified Full length Not specified Full length 11717445
DED Caspase8 Link FADD Fluorescence resonance energy transfer HEK293 Not specified Full length Not specified Not specified 11717445
(Link: click this icon to show interactions only between the two corresponding DDs)