J Biol Chem. 2002 Mar 1;277(9):7501-8. Epub 2001 Dec 11.

Identification and characterization of DEDD2, a death effector domain-containing protein.External

Roth, W., Stenner-Liewen, F., Pawlowski, K., Godzik, A., Reed, J. C.,
--- - The Burnham Institute, 10901 N Torrey Pines Road, La Jolla, CA 92037, USA.
A novel Death Effector Domain-containing protein was identified, DEDD2, which is closest in amino acid sequence homology to death effector domain-containing DNA-binding protein, DEDD. DEDD2 mRNA is expressed widely in adult human tissues with highest levels in liver, kidney, and peripheral blood leukocytes. DEDD2 interacts with FLIP, but not with Fas-associated death domain (FADD) or caspase-8. Overexpression of DEDD2 induces moderate apoptosis and results in substantial sensitization to apoptosis induced by Fas (CD95/APO-1), tumor necrosis factor-related apoptosis-inducing ligand (TRAIL, Apo2L), or FADD. In contrast, Bax- or staurosporine-mediated cell death is not affected by expression of DEDD2. Fluorescence microscopy showed that overexpressed DEDD2 translocates to the nucleus, which is dependent on the presence of a bipartite nuclear localization signal in the DEDD2 protein. Mutagenesis studies revealed that the translocation of the DED of DEDD2 to the nucleus is essential for its pro-apoptotic activity. These findings suggest that DEDD2 is involved in the regulation of nuclear events mediated by the extrinsic apoptosis pathway.
PMID: 11741985External
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DED DEDD2 Link FLIP GST fusion protein pull-down Human Not specified E.coli Not specified Not specified In vitro translation 11741985
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DED Caspase8 Link DEDD GST fusion protein pull-down HEK293 Not specified Not specified Not specified Not specified 11741985
DED DEDD Link FADD GST fusion protein pull-down HEK293 Not specified Not specified Not specified Not specified 11741985
DED DEDD Link FLIP GST fusion protein pull-down HEK293 Not specified Not specified Not specified Not specified 11741985
DED DEDD2 Link FLIP Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 11741985
(Link: click this icon to show interactions only between the two corresponding DDs)