FEBS Lett. 2002 Sep 11;527(1-3):250-4.

Identification of a basic surface area of the FADD death effector domain critical for apoptotic signaling.External

Kaufmann, M., Bozic, D., Briand, C., Bodmer, J. L., Zerbe, O., Kohl, A., Tschopp, J., Grutter, M. G.,
--- - Institute of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH-8057, Zurich, Switzerland.
Death effector domains (DEDs) are protein-protein interaction domains found in the death inducing signaling complex (DISC). Performing a structure-based alignment of all DED sequences we identified a region of high diversity in alpha-helix 3 and propose a classification of DEDs into class I DEDs typically containing a stretch of basic residues in the alpha-helix 3 region whereas DEDs of class II do not. Functional assays using mutants of Fas-associated death domain revealed that this basic region influences binding and recruitment of caspase-8 and cellular FLICE inhibitor protein to the DISC.
PMID: 12220669External
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DED Caspase8 Link FADD Co-immunoprecipitation HEK293 Human 2-177 Human 2-208 12220669
DED FADD Link FLIP Co-immunoprecipitation HEK293 Human 2-208 Human 1-171 12220669
DED FADD Link FLIP Co-immunoprecipitation HEK293 Human 2-208 Human Full length 12220669
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
DED Caspase8 Link FADD Co-immunoprecipitation K33Q and K35Q, F25G (FADD) 12220669
DED FADD Link FLIP Co-immunoprecipitation K33Q and K35Q, F25G, F25Y, K33N and K35N (FADD) 12220669
(Link: click this icon to show interactions only between the two corresponding DDs)