Biochem Biophys Res Commun. 2003 Apr 4;303(2):525-31.

Characterization of a cascade of protein interactions initiated at the IL-1 receptor.External

Boch, J. A., Yoshida, Y., Koyama, Y., Wara-Aswapati, N., Peng, H., Unlu, S., Auron, P. E.,
--- - Department of Medicine, Harvard Medical School, and the Beth Israel Deaconess Medical Center, Boston, MA, USA.
Prior studies have identified molecules involved in IL-1 signaling that transmit the extracellular stimulus to downstream kinase molecules causing altered transcriptional activity. Many of these investigations have relied heavily on ligand induced protein-protein interactions detected by immuno-coprecipitation to map the cascade of events from receptor binding to activation of downstream signaling intermediates. Direct protein interactions have not been commonly reported. An in vitro study was undertaken to better define the direct protein-protein interactions involved in IL-1 signaling. Results indicate that IRAK2 is capable of direct association with either IL-1R(I) or IL-1R(AcP). IRAK2 is also capable of associating directly with MyD88 by distinct regions. Finally, IRAK2 interactions with TRAF6 were mapped and demonstrate differences from more proximal signaling intermediates. A model is presented that reflects the specific molecular interactions responsible for recruiting signaling intermediates to the IL-1 receptor cytoplasmic domains.
PMID: 12659850External
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DD IRAK2 Link Myd88 GST fusion protein pull-down Not specified Full length In vitro translation Not specified 1-296 E.coli 12659850
DD IRAK2 Link Myd88 GST fusion protein pull-down Not specified Full length In vitro translation Not specified 1-109 E.coli 12659850
DD IRAK2 Link Myd88 GST fusion protein pull-down Not specified Full length In vitro translation Not specified 1-155 E.coli 12659850
(Link: click this icon to show interactions only between the two corresponding DDs)