J Neurochem. 2003 Sep;86(5):1260-70.

Expression of death-associated protein kinase and recruitment to the tumor necrosis factor signaling pathway following brief seizures.External

Henshall, D. C., Araki, T., Schindler, C. K., Shinoda, S., Lan, J. Q., Simon, R. P.,
--- - Robert S. Dow Neurobiology Laboratories, Legacy Clinical Research & Technology Center, 1225 NE 2nd Avenue, Portland, OR 97232, USA. dhenshall@DowNeurobiology.org
Death-associated protein (DAP) kinase is calcium-regulated and known to function downstream of death receptors, prompting us to examine its role in the mechanism of seizure-induced neuronal death. Brief seizures were focally evoked in rats, eliciting neuronal death within the CA3 subfield of the hippocampus, and to a lesser extent, cortex. Western blotting confirmed expression of DAP kinase within hippocampus and cortex at the predicted weight of approximately 160 kDa. Immunohistochemistry revealed seizures triggered a significant increase in numbers of DAP kinase-expressing cells within CA3 and cortex, without affecting cell counts within seizure-resistant CA2 or the dentate gyrus. Numbers of DAP kinase-expressing cells were increased in relation to specific patterns of injury-causing seizure activity, electrographically defined. Seizures caused an early increase in DAP kinase binding to actin, and association with calmodulin. Co-immunoprecipitation studies also revealed seizures triggered binding of DAP kinase to the tumor necrosis factor receptor 1 and the Fas-associated death domain protein, commensurate with caspase-8 proteolysis. In contrast, within surviving fields of the hippocampus, DAP kinase interacted with the molecular chaperone 14-3-3. These data suggest DAP kinase is involved in the molecular pathways activated during seizure-induced neuronal death.
PMID: 12911633External
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DD DAPK1 Link FADD Co-Immunofluorescence staining Rat brain cell 12911633
DD DAPK1 Link FADD Co-immunoprecipitation Rat brain cell 12911633
DD DAPK1 Link TNFR1 Co-Immunofluorescence staining Rat brain cell 12911633
DD DAPK1 Link TNFR1 Co-immunoprecipitation Rat brain cell 12911633
(Link: click this icon to show interactions only between the two corresponding DDs)