FEBS Lett. 2004 Dec 17;578(3):239-44.

Inhibition of Bcl10-mediated activation of NF-kappa B by BinCARD, a Bcl10-interacting CARD protein.External

Woo, H. N., Hong, G. S., Jun, J. I., Cho, D. H., Choi, H. W., Lee, H. J., Chung, C. W., Kim, I. K., Jo, D. G., Pyo, J. O., Bertin, J., Jung, Y. K.,
--- - Department of Life Science, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of Korea.
We have identified a novel CARD-containing protein from EST database. BinCARD (Bcl10-interacting protein with CARD). BinCARD was ubiquitously expressed. Co-immunoprecipitation, In vitro binding, mammalian two-hybrid, and immunostaining assays revealed that BinCARD interacted with Bcl10 through CARD. BinCARD potently suppressed NF-kappa B activation induced by Bcl10 and decreased the amounts of phosphorylated Bcl10. Mutations at the residue Leu17 or Leu65, which is highly conserved in CARD, abolished the inhibitory effects of BinCARD on both Bcl10-induced activation of NF-kappa B and phosphorylation of Bcl10. Further, expression of BinCARD inhibited Bcl10 phosphorylation induced by T cell activation signal. These results suggest that BinCARD interacts with Bcl10 to inhibit Bcl10-mediated activation of NF-kappa B and to suppress Bcl10 phosphorylation.
PMID: 15637807External
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
CARD Bcl-10 Link BinCARD GST fusion protein pull-down Not specified Not specified E.coli Human Not specified In vitro translation 15637807
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Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
CARD Bcl-10 Link BinCARD Co-Immunofluorescence staining HeLa Not specified Not specified Human Not specified 15637807
CARD Bcl-10 Link BinCARD Co-immunoprecipitation HEK293 Not specified Not specified Human Not specified 15637807
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
CARD Bcl-10 Link BinCARD GST fusion protein pull-down L65A (BinCARD) 15637807
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
CARD Bcl-10 Link BinCARD Luciferase assay NF-kB activation block 15637807
(Link: click this icon to show interactions only between the two corresponding DDs)