Nat Immunol. 2005 Oct;6(10):981-8. Epub 2005 Aug 28.

IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction.External

Kawai, T., Takahashi, K., Sato, S., Coban, C., Kumar, H., Kato, H., Ishii, K. J., Takeuchi, O., Akira, S.,
--- - Exploratory Research for Advanced Technology, Japan Science and Technology Agency.
Type I interferons are central mediators for antiviral responses. Using high-throughput functional screening of interferon inducers, we have identified here a molecule we call interferon-beta promoter stimulator 1 (IPS-1). Overexpression of IPS-1 induced type I interferon and interferon-inducible genes through activation of IRF3, IRF7 and NF-kappaB transcription factors. TBK1 and IKKi protein kinases were required for the IPS-1-mediated interferon induction. IPS-1 contained an N-terminal CARD-like structure that mediated interaction with the CARD of RIG-I and Mda5, which are cytoplasmic RNA helicases that sense viral infection. 'Knockdown' of IPS-1 by small interfering RNA blocked interferon induction by virus infection. Thus, IPS-1 is an adaptor involved in RIG-I- and Mda5-mediated antiviral immune responses.
PMID: 16127453External
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
CARD MAVS Link MDA5 Co-immunoprecipitation HEK293 Human Not specified Human 1-575 16127453
CARD MAVS Link RIG-1 Co-immunoprecipitation HEK293 Human Not specified Human 1-604 16127453
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
CARD MAVS Link MDA5 ELISA Type 1 interferon induced 16127453
CARD MAVS Link RIG-1 ELISA Type 1 interferon induced 16127453
(Link: click this icon to show interactions only between the two corresponding DDs)