EMBO Rep. 2009 Jun;10(6):642-8. Epub 2009 May 15.

COP9 signalosome controls the Carma1-Bcl10-Malt1 complex upon T-cell stimulation.External

Welteke, V., Eitelhuber, A., Duwel, M., Schweitzer, K., Naumann, M., Krappmann, D.,
--- - Department Cellular Signal Integration, Helmholtz Zentrum Munchen-German Research Center for Environmental Health, Institute of Toxicology, Ingolstadter Landstrasse 1, Neuherberg 85764, Germany.
The Carma1-Bcl10-Malt1 (CBM) complex connects T-cell receptor (TCR) signalling to the canonical IkappaB kinase (IKK)/NF (nuclear factor)-kappaB pathway. Earlier studies have indicated that the COP9 signalosome (CSN), a pleiotropic regulator of the ubiquitin/26S proteasome system, controls antigen responses in T cells. The CSN is required for the degradation of the NF-kappaB inhibitor IkappaBalpha, but other molecular targets involved in T-cell signalling remained elusive. Here, we identify the CSN subunit 5 (CSN5) as a new interactor of Malt1 and Carma1. T-cell activation triggers the recruitment of the CSN to the CBM complex, and CSN downregulation impairs TCR-induced IKK activation. Furthermore, the CSN is required for maintaining the stability of Bcl10 in response to T-cell activation. Taken together, our data provide evidence for a functional link between the evolutionarily conserved CSN and the adaptive immunoregulatory CBM complex in T cells.
PMID: 19444310External
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
CARD Bcl-10 Link CARD11 Co-immunoprecipitation Jurkat 19444310
(Link: click this icon to show interactions only between the two corresponding DDs)