Nat Immunol. 2010 Oct;11(10):912-9. Epub 2010 Sep 5.

Caspase-12 controls West Nile virus infection via the viral RNA receptor RIG-I.External

Wang, P., Arjona, A., Zhang, Y., Sultana, H., Dai, J., Yang, L., LeBlanc, P. M., Doiron, K., Saleh, M., Fikrig, E.,
--- - Section of Infectious Diseases, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut, USA.
Caspase-12 has been shown to negatively modulate inflammasome signaling during bacterial infection. Its function in viral immunity, however, has not been characterized. We now report an important role for caspase-12 in controlling viral infection via the pattern-recognition receptor RIG-I. After challenge with West Nile virus (WNV), caspase-12-deficient mice had greater mortality, higher viral burden and defective type I interferon response compared with those of challenged wild-type mice. In vitro studies of primary neurons and mouse embryonic fibroblasts showed that caspase-12 positively modulated the production of type I interferon by regulating E3 ubiquitin ligase TRIM25-mediated ubiquitination of RIG-I, a critical signaling event for the type I interferon response to WNV and other important viral pathogens.
PMID: 20818395External
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
CARD Caspase12 Link RIG-1 Co-Immunofluorescence staining Mouse embryonic fibroblast Not specified Not specified Human Not specified 20818395
CARD Caspase12 Link RIG-1 GST fusion protein pull-down Mouse embryonic fibroblast Not specified Not specified Human Not specified 20818395
(Link: click this icon to show interactions only between the two corresponding DDs)