J Biol Chem. 1995 Apr 7;270(14):7795-8.

A novel protein that interacts with the death domain of Fas/APO1 contains a sequence motif related to the death domain.External

Boldin, M. P., Varfolomeev, E. E., Pancer, Z., Mett, I. L., Camonis, J. H., Wallach, D.,
--- - Department of Membrane Research and Biophysics, Weizmann Institute of Science, Rehovot, Israel.
Signaling for cell death by Fas/APO1 occurs via a distinct region in its intracellular domain. This region contains a conserved sequence motif, the death domain motif, that is also found in the intracellular domains of the p55 tumor necrosis factor receptor and the low affinity nerve growth factor receptor, as well as in the regulatory domain of the ankyrins. A novel protein that specifically binds to the death domain of Fas/APO1 but not to Fas/APO1 molecules with a loss of function point mutation occurring in lprcg mice was cloned by a two-hybrid screen of a HeLa cells' cDNA library. The cloned protein itself contains a death domain motif, and this region binds to the death domain of Fas/APO1, while the region upstream to the death domain prompts self-association of the protein. Induced expression of the protein results in ligand-independent triggering of cytotoxicity, suggesting that it is involved in cell death induction by Fas/APO1.
PMID: 7536190External
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DD FADD Link Fas GST fusion protein pull-down Not specified Not specified Not specified Human 175-319 Not specified 7536190
DD FADD Link Fas Yeast two-hybrid Not specified 130-245 Yeast Human 175-319 Yeast 7536190
DD FADD Link Fas Yeast two-hybrid Not specified 130-245 Yeast Human 200-319 Yeast 7536190
DD Fas Link Fas Yeast two-hybrid Human 175-319 Yeast Human 175-319 Yeast 7536190
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DD FADD Link Fas Co-immunoprecipitation HeLa Not specified Not specified Not specified Not specified 7536190
(Link: click this icon to show interactions only between the two corresponding DDs)