Cell. 1995 May 19;81(4):505-12.

FADD, a novel death domain-containing protein, interacts with the death domain of Fas and initiates apoptosis.External

Chinnaiyan, A. M., O'Rourke, K., Tewari, M., Dixit, V. M.,
--- - Department of Pathology, University of Michigan Medical School, Ann Arbor 48109, USA.
Using the cytoplasmic domain of Fas in the yeast two-hybrid system, we have identified a novel interacting protein, FADD, which binds Fas and Fas-FD5, a mutant of Fas possessing enhanced killing activity, but not the functionally inactive mutants Fas-LPR and Fas-FD8. FADD contains a death domain homologous to the death domains of Fas and TNFR-1. A point mutation in FADD, analogous to the lpr mutation of Fas, abolishes its ability to bind Fas, suggesting a death domain to death domain interaction. Overexpression of FADD in MCF7 and BJAB cells induces apoptosis, which, like Fas-induced apoptosis, is blocked by CrmA, a specific inhibitor of the interleukin-1 beta-converting enzyme. These findings suggest that FADD may play an important role in the proximal signal transduction of Fas.
PMID: 7538907External
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DD FADD Link Fas GST fusion protein pull-down Not specified Not specified Yeast Not specified Not specified Yeast 7538907
DD FADD Link Fas In vitro purification protein assembly(Pull down) Not specified Not specified HEK293 Not specified 1-304 E.coli 7538907
DD FADD Link Fas Yeast two-hybrid Not specified Not specified In vitro translation Not specified 1-304 E.coli 7538907
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DD FADD Link Fas Co-immunoprecipitation HEK293 Not specified Not specified Not specified 1-304 7538907
(Link: click this icon to show interactions only between the two corresponding DDs)