Cell. 1995 May 19;81(4):495-504.

The TNF receptor 1-associated protein TRADD signals cell death and NF-kappa B activation.External

Hsu, H., Xiong, J., Goeddel, D. V.,
--- - Tularik, Incorporated, South San Francisco, California 94080, USA.
Many diverse activities of tumor necrosis factor (TNF) are signaled through TNF receptor 1 (TNFR1). We have identified a novel 34 kDa protein, designated TRADD, that specifically interacts with an intracellular domain of TNFR1 known to be essential for mediating programmed cell death. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa B. The C-terminal 118 amino acids of TRADD are sufficient to trigger both of these activities and likewise sufficient for interaction with the death domain of TNFR1. TRADD-mediated cell death can be suppressed by the crmA gene, which encodes a specific inhibitor of the interleukin-1 beta-converting enzyme. However, NF-kappa B activation by TRADD is not inhibited by crmA expression, demonstrating that the signaling pathways for TNF-induced cell death and NF-kappa B activation are distinct.
PMID: 7758105External
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DD TNFR1 Link TRADD GST fusion protein pull-down Human Not specified Not specified Human Full length In vitro translation 7758105
DD TNFR1 Link TRADD GST fusion protein pull-down Human Not specified Not specified Human 1-305 In vitro translation 7758105
DD TNFR1 Link TRADD GST fusion protein pull-down Human Not specified Not specified Human 195-312 In vitro translation 7758105
DD TNFR1 Link TRADD Yeast two-hybrid Human 214-426 Yeast Human Full length Yeast 7758105
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DD TNFR1 Link TRADD Co-immunoprecipitation HEK293 Human Not specified Human Not specified 7758105
(Link: click this icon to show interactions only between the two corresponding DDs)