J Biol Chem. 1996 Mar 1;271(9):4961-5.

FADD/MORT1 is a common mediator of CD95 (Fas/APO-1) and tumor necrosis factor receptor-induced apoptosis.External

Chinnaiyan, A. M., Tepper, C. G., Seldin, M. F., O'Rourke, K., Kischkel, F. C., Hellbardt, S., Krammer, P. H., Peter, M. E., Dixit, V. M.,
--- - Department of Pathology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA.
CD95 (Fas/APO-1) and tumor necrosis factor receptor-1 (TNFR-1) are related molecules that signal apoptosis. Recently, a number of novel binding proteins have been proposed to mediate the signaling of these death receptors. Here we report that an N-terminal truncation of one of these candidate signal transducers, FADD/MORT1, abrogates CD95-induced apoptosis, ceramide generation, and activation of the cell death protease Yama/CPP32. In addition, this dominant-negative derivative of FADD (FADD-DN) blocked TNF-induced apoptosis while not affecting NF- kappaB activation. FADD-DN bound both receptors, and in the case of CD95, it disrupted the assembly of a signaling complex. Taken together, our results functionally establish FADD as the apoptotic trigger of CD95 and TNFR-1.
PMID: 8617770External
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DD FADD Link Fas Co-immunoprecipitation HEK293 Not specified 80-208 Not specified Not specified 8617770
DD FADD Link Fas Co-immunoprecipitation HEK293 Not specified 2-208 Not specified Not specified 8617770
DD FADD Link TNFR1 Co-immunoprecipitation HEK293 Not specified 2-208 Not specified Not specified 8617770
DD FADD Link TNFR1 Co-immunoprecipitation HEK293 Not specified 80-208 Not specified Not specified 8617770
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DD FADD Link Fas MTT assay Apoptosis (DISC) 8617770
DD FADD Link TNFR1 MTT assay Apoptosis (DISC) 8617770
(Link: click this icon to show interactions only between the two corresponding DDs)