J Exp Med. 1996 Mar 1;183(3):1271-5.

A potential mechanism of "cross-talk" between the p55 tumor necrosis factor receptor and Fas/APO1: proteins binding to the death domains of the two receptors also bind to each other.External

Varfolomeev, E. E., Boldin, M. P., Goncharov, T. M., Wallach, D.,
--- - Department of Membrane Research and Biophysics, The Weizmann Institute of Science, Rehovot, Israel.
The p55 tumor necrosis factor (TNF) receptor and Fas/APO1 induce cell death via distinct regions in their intracellular domains. Three cytoplasmic proteins that bind to these receptor regions have been identified recently. One, MORT1 (also called FADD), binds to Fas/APO1 but not to p55-R; another, TRADD, binds to the p55 TNF receptor but not to Fas/APO1; and the third, RIP, binds weakly to both receptors. The regions within these proteins that are involved in binding to the receptors and the receptor regions to which they bind share a common sequence motif, that of the "death domain." This study shows that the death domain motifs in MORT1, TRADD, and RIP bind effectively to each other, a mode of binding that may allow "cross-talk" between the functional expression of the p55 TNF receptor and Fas/APO1.
PMID: 8642271External
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DD FADD Link Fas Yeast two-hybrid Not specified 92-208 Yeast Not specified 210-319 Yeast 8642271
DD FADD Link RIP Yeast two-hybrid Not specified 92-208 Yeast Not specified 261-372 Yeast 8642271
DD FADD Link TNFR1 Yeast two-hybrid Not specified 92-208 Yeast Not specified 326-426 Yeast 8642271
DD FADD Link TRADD Yeast two-hybrid Not specified 92-208 Yeast Not specified 195-312 Yeast 8642271
DD Fas Link Fas Yeast two-hybrid Not specified 210-319 Yeast Not specified 210-319 Yeast 8642271
DD Fas Link RIP Yeast two-hybrid Not specified 210-319 Yeast Not specified 261-372 Yeast 8642271
DD RIP Link RIP Yeast two-hybrid Not specified 261-372 Yeast Not specified 261-372 Yeast 8642271
DD RIP Link TNFR1 Yeast two-hybrid Not specified 261-372 Yeast Not specified 326-426 Yeast 8642271
DD RIP Link TRADD Yeast two-hybrid Not specified 261-372 Yeast Not specified 195-312 Yeast 8642271
DD TNFR1 Link TNFR1 Yeast two-hybrid Not specified 326-426 Yeast Not specified 326-426 Yeast 8642271
DD TNFR1 Link TRADD Yeast two-hybrid Not specified 326-426 Yeast Not specified 195-312 Yeast 8642271
DD TRADD Link TRADD Yeast two-hybrid Not specified 195-312 Yeast Not specified 195-312 Yeast 8642271
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DD FADD Link RIP Co-immunoprecipitation HeLa Not specified 19-753 Not specified 261-372 8642271
DD FADD Link TRADD Co-immunoprecipitation HeLa Not specified 19-753 Not specified 195-312 8642271
DD RIP Link TRADD Co-immunoprecipitation HeLa Not specified 261-372 Not specified 195-312 8642271
(Link: click this icon to show interactions only between the two corresponding DDs)