Nature. 1996 Nov 28;384(6607):372-5.

A death-domain-containing receptor that mediates apoptosis.External

Kitson, J., Raven, T., Jiang, Y. P., Goeddel, D. V., Giles, K. M., Pun, K. T., Grinham, C. J., Brown, R., Farrow, S. N.,
--- - Cell Biology Unit, Glaxo-Wellcome Medicines Research Centre, Stevenage, Hertfordshire SG1 2NY, UK.
The cell-killing effects of the cytokines TNF-alpha and FasL are mediated by the distinct cell-surface receptors TNFR1, TNFR2 and Fas (also known as CD95/APO-1), which are all members of a receptor superfamily that is important for regulating cell survival. The cytoplasmic regions of TNFR1 and Fas contain a conserved 'death' domain which is an essential component of the signal pathway that triggers apoptosis and activation of the transcription factor NF-kappaB (refs 5,6). Here we report the isolation of a 54K receptor that is a new member of the TNFR superfamily, using the death domain of TNFR1 in a yeast two-hybrid system. This protein, WSL-1, is most similar to TNFR1 itself, particularly in the death-domain region. The gene wsl-1 is capable of inducing apoptosis when transfected into 3T3 and 293 cells, and can also activate NF-kappaB in 293 cells. Like TNFR1, WSL-1 will homodimerize in yeast. WSL-1 also interacts specifically with the TNFR1-associated molecule TRADD. The tissue distribution is very restricted and significantly different from that of Fas and TNFR1.
PMID: 8934525External
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DD DR3 Link DR3 Yeast two-hybrid Not specified Not specified Yeast Not specified Not specified Yeast 8934525
DD DR3 Link TNFR1 Yeast two-hybrid Not specified Not specified Yeast Human Not specified Yeast 8934525
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DD DR3 Link TRADD Co-immunoprecipitation HEK293 Not specified Not specified Not specified Not specified 8934525
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Characterization
  Stoichiometry Affinity Protein-Protein interface Reference
Family DD1 DD2 Method Mutation Complex structure
DD DR3 Link DR3 Yeast two-hybrid L354A, L356A, D373A (DR3) 8934525
DD DR3 Link TNFR1 Yeast two-hybrid L354A, L356A, D373A (DR3) 8934525
(Link: click this icon to show interactions only between the two corresponding DDs)