Immunity. 1997 Jun;6(6):751-63.

Casper is a FADD- and caspase-related inducer of apoptosis.External

Shu, H. B., Halpin, D. R., Goeddel, D. V.,
--- - Tularik, Incorporated, South San Francisco, California 94080, USA.
Caspases are cysteine proteases that play a central role in apoptosis. Caspase-8 may be the first enzyme of the proteolytic cascade activated by the Fas ligand and tumor necrosis factor (TNF). Caspase-8 is recruited to Fas and TNF receptor-1 (TNF-R1) through interaction of its prodomain with the death effector domain (DED) of the receptor-associating FADD. Here we describe a novel 55 kDa protein, Casper, that has sequence similarity to caspase-8 throughout its length. However, Casper is not a caspase since it lacks several conserved amino acids found in all caspases. Casper interacts with FADD, caspase-8, caspase-3, TRAF1, and TRAF2 through distinct domains. When overexpressed in mammalian cells, Casper potently induces apoptosis. A C-terminal deletion mutant of Casper inhibits TNF- and Fas-induced cell death, suggesting that Casper is involved in these apoptotic pathways.
PMID: 9208847External
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DED Caspase10 Link FLIP Yeast two-hybrid Human Full length Yeast Human 1-215 Yeast 9208847
DED Caspase8 Link FLIP Yeast two-hybrid Human Full length Yeast Human 1-215 Yeast 9208847
DED FADD Link FLIP Yeast two-hybrid Human Full length Yeast Human 1-215 Yeast 9208847
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DED Caspase8 Link FLIP Co-immunoprecipitation HEK293 Not specified Not specified Human Full length 9208847
DED FADD Link FLIP Co-immunoprecipitation HEK293 Not specified Not specified Human 1-195 9208847
DED FADD Link FLIP Co-immunoprecipitation HEK293 Not specified Not specified Human 78-480 9208847
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DED FADD Link FLIP β-galactosidase staining Apoptosis inhibition 9208847
(Link: click this icon to show interactions only between the two corresponding DDs)