J Biol Chem. 1997 Jul 25;272(30):18542-5.

FLAME-1, a novel FADD-like anti-apoptotic molecule that regulates Fas/TNFR1-induced apoptosis.External

Srinivasula, S. M., Ahmad, M., Ottilie, S., Bullrich, F., Banks, S., Wang, Y., Fernandes-Alnemri, T., Croce, C. M., Litwack, G., Tomaselli, K. J., Armstrong, R. C., Alnemri, E. S.,
--- - Center for Apoptosis Research and the Department of Microbiology and Immunology, Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
We identified and cloned a novel human protein that contains FADD/Mort1 death effector domain homology regions, designated FLAME-1. FLAME-1, although most similar in structure to Mch4 and Mch5, does not possess caspase activity but can interact specifically with FADD, Mch4, and Mch5. Interestingly, FLAME-1 is recruited to the Fas receptor complex and can abrogate Fas/TNFR-induced apoptosis upon expression in FasL/tumor necrosis factor-sensitive MCF-7 cells, possibly by acting as a dominant-negative inhibitor. These findings identify a novel endogenous control point that regulates Fas/TNFR1-mediated apoptosis.
PMID: 9228018External
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vitro interaction
  DD1 DD2 Reference
Family DD1 DD2 Method Species Region Expression Species Region Expression
DED Caspase10 Link FLIP GST fusion protein pull-down Not specified Not specified In vitro translation Human Full length Not specified 9228018
DED Caspase10 Link FLIP Yeast two-hybrid Not specified 18-189 Yeast Human 1-160 Yeast 9228018
DED Caspase8 Link FLIP GST fusion protein pull-down Not specified 1-201 In vitro translation Human Full length Not specified 9228018
DED Caspase8 Link FLIP Yeast two-hybrid Not specified 1-201 Yeast Human 1-160 Yeast 9228018
DED FADD Link FLIP GST fusion protein pull-down Not specified 1-79 In vitro translation Human Full length Not specified 9228018
DED FADD Link FLIP Yeast two-hybrid Not specified 1-79 Yeast Human 1-160 Yeast 9228018
DED FLIP Link FLIP Yeast two-hybrid Human 1-160 Yeast Human 1-160 Yeast 9228018
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DED Caspase10 Link FLIP Co-immunoprecipitation HEK293 Not specified 18-189 Human Full length 9228018
DED Caspase8 Link FLIP Co-immunoprecipitation HEK293 Not specified 1-201 Human Full length 9228018
DED FADD Link FLIP Co-immunoprecipitation HEK293 Not specified Not specified Human Full length 9228018
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DED Caspase10 Link FLIP β-galactosidase staining Apoptosis inhibition 9228018
DED Caspase8 Link FLIP β-galactosidase staining Apoptosis inhibition 9228018
(Link: click this icon to show interactions only between the two corresponding DDs)