Immunity. 1997 Dec;7(6):821-30.

Death receptor 5, a new member of the TNFR family, and DR4 induce FADD-dependent apoptosis and activate the NF-kappaB pathway.External

Chaudhary, P. M., Eby, M., Jasmin, A., Bookwalter, A., Murray, J., Hood, L.,
--- - Department of Molecular Biotechnology, University of Washington, Seattle 98195, USA.
Death receptor 4 (DR4) is a recently described receptor for the cytotoxic ligand TRAIL that reportedly uses a FADD-independent pathway to induce apoptosis and does not activate the NF-kappaB pathway. We have isolated a new member of the tumor necrosis factor receptor (TNFR) family, designated DR5, which bears a high degree of sequence homology to DR4. However, contrary to the previous reports, both DR4- and DR5-induced apoptosis can be blocked by dominant-negative FADD, and both receptors can activate NF-kappaB using a TRADD-dependent pathway. Finally, both receptors can interact with FADD, TRADD, and RIP. Thus, both DR5 and DR4 use FADD, TRADD, and RIP in their signal transduction pathways, and FADD is the common mediator of apoptosis by all known death domain-containing receptors.
PMID: 9430227External
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
In vivo interaction
  Endogenous
expression
Overexpression DD1 DD2 Reference
Family DD1 DD2 Method Species Region Species Region
DD DR3 Link FADD Co-immunoprecipitation HEK293 Human 27-418 Human Not specified 9430227
DD DR3 Link TRADD Co-immunoprecipitation HEK293 Human Not specified Not specified Not specified 9430227
DD DR4 Link FADD Co-immunoprecipitation HEK293 Human 106-468 Human Not specified 9430227
DD DR4 Link RIP His fusion protein pull-down HEK293 Human 106-468 Human Not specified 9430227
DD DR4 Link TRADD Co-immunoprecipitation HEK293 Human 106-468 Human 121-312 9430227
DD DR5 Link FADD Co-immunoprecipitation HEK293 Human 51-411 Human Not specified 9430227
DD DR5 Link RIP Co-immunoprecipitation HEK293 Human 51-411 Human Not specified 9430227
DD DR5 Link TRADD Co-immunoprecipitation HEK293 Human 51-411 Human 121-312 9430227
DD FADD Link Fas Co-immunoprecipitation HEK293 Human Not specified Human 19-335 9430227
(Link: click this icon to show interactions only between the two corresponding DDs)
Arrow2 In vitro interaction Arrow2 In vivo interaction Arrow2 Characterization Arrow2 Functional role Arrow2 top
Functional Role
Family DD1 DD2 Method Death-related Death-unrelated Reference
DD DR4 Link FADD β-galactosidase staining Apoptosis (DISC) 9430227
DD DR4 Link TRADD Luciferase assay NF-kB activation 9430227
DD DR5 Link FADD β-galactosidase staining Apoptosis (DISC) 9430227
DD DR5 Link TRADD Luciferase assay NF-kB activation 9430227
(Link: click this icon to show interactions only between the two corresponding DDs)